Aspergillus fumigatus is a green echinulate with greenish phialides and 2.5-3 mm conidia. The diverse biological functions of A. fumigatus secondary metabolites make them interesting. The ethyl acetate extract of A. fumigatus was tested for antibacterial activity. Culture media, temperature, incubation and pH were optimized for A. fumigatus growth. Continuous 150rpm agitation incubated the fungus at 28°C for 10 days. Potato Dextrose Broth at 28°C in shaking incubator at pH 04 produced the most biomass and secondary metabolites. Metabolite antibacterial activity was tested. Salmonella flexneri had the greatest zone of inhibition at 100μl (25.66mm) while Staphylococcus aureus had the least (16.33mm). At 75μg/mL, S. flexneri showed 23.66mm activity and S. typhi 14.66mm. At 50μg/mL, S. flexneri was 21.33mm and S. typhi 12.33mmMBC was 0.01μg/μl and MIC50 varied. At 100μg/mL, the metabolites showed antifungal efficacy against Penicillium chrysogenum (26.33mm) but not A. flavus (21.33mm). A. oryzae was significantly inhibited at 75μg/mL (26.33mm) and 50μg/mL (20.33mm). 1000μl demonstrated 100% phytotoxicity, 100μl 60% and 10μl 50%. Bactrocera cucurbitae, Sitotroga cerealella and Callosobruchus maculatus were killed at 150, 100 and 75μl. Metabolites and antibiotics synergized well. Metabolites have alkanes, esters and ethers in their infrared spectra.