Interaction of Cibacron Blue F3GA with Escherichia coli DNA polymerase I and with T4 DNA polymerase

Biochim Biophys Acta. 1979 Apr 26;562(2):231-9. doi: 10.1016/0005-2787(79)90169-2.

Abstract

Individual rapid procedures for the enrichment of Escherichia coli DNA polymerase I and of bacteriophage T4 DNA polymerase free of endonuclease activity are described using Blue dextran-Sepharose chromatography. The blue dye of Blue dextran-Sepharose selectively binds to the deoxynucleoside triphosphate substrate site of the E. coli but not the T4 enzyme indicating that the catalytic sites of these two enzymes which catalyze the same polymerization reaction in vitro are quite distinct.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anthracenes* / pharmacology
  • Chromatography, Affinity
  • Coliphages / enzymology*
  • Coloring Agents* / pharmacology
  • DNA Polymerase I / isolation & purification*
  • DNA Polymerase I / metabolism
  • DNA-Directed DNA Polymerase / isolation & purification*
  • DNA-Directed DNA Polymerase / metabolism
  • Deoxyribonucleases / isolation & purification
  • Endonucleases / isolation & purification
  • Escherichia coli / enzymology*
  • Kinetics
  • Triazines / pharmacology

Substances

  • Anthracenes
  • Coloring Agents
  • Triazines
  • DNA Polymerase I
  • DNA-Directed DNA Polymerase
  • Deoxyribonucleases
  • Endonucleases