Quality traits analysis of 153 wheat lines derived from CIMMYT and China

Pengpeng Liu; Zhe Zhang; Yuruo Yin; Shanshan Yan; Yong Ren; Wei Sang; Hongjun Xu; Xinnian Han; Fengjuan Cui; Yingbin Nie; Dezhen Kong; Wei Li; Caixia Lan; Peiyuan Mu

doi:10.3389/fgene.2023.1198835

Quality traits analysis of 153 wheat lines derived from CIMMYT and China

Front Genet. 2023 Aug 2:14:1198835. doi: 10.3389/fgene.2023.1198835. eCollection 2023.

Authors

Pengpeng Liu¹, Zhe Zhang², Yuruo Yin², Shanshan Yan², Yong Ren³, Wei Sang¹, Hongjun Xu¹, Xinnian Han¹, Fengjuan Cui¹, Yingbin Nie¹, Dezhen Kong¹, Wei Li¹, Caixia Lan², Peiyuan Mu¹

Affiliations

¹ Institute of Crop Research, Xinjiang Academy of Agri-Reclamation Sciences, Key Lab of Xinjiang Production and Construction Corps for Cereal Quality Research and Genetic Improvement, Shihezi, Xinjiang, China.
² National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.
³ Mianyang Institute of Agricultural Science, Crop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province, Mianyang City, Sichuan, China.

Abstract

In order to understand the difference of quality for Chinese and CIMMYT wheat varieties (lines), we selected 153 wheat germplasm from both China and CIMMYT to explore the contribution relationship of different allelic variation combinations to wheat quality through genotyping and phenotyping, including grain hardness, polyphenol oxidase (PPO) activity, lipoxygenase (LOX) activity, yellow pigment (YP) content and protein content. In terms of flour milling quality, Chinese wheat varieties were mainly carrying Pina-D1a/Pinb-D1b, accounting for 32.0% of the total tested varieties, while the CIMMYT wheat lines were mainly carrying Pina-D1b/Pinb-D1a with 45.8% of the total collection. The distribution frequencies of subunit 1/2* and 5 + 10 were 47.0% and 42.5%, respectively, in CIMMYT varieties, however they were only 31.4% and 13.7% respectively of the Chinese wheat tested varieties. In addition, the proportion of phytoene synthase (PSY) allele, PPO allele and LOX active allele were roughly the same between Chinese and CIMMYT varieties. Based on the present study, we found that Pina gene had a greater impact on grain hardness value than Pinb gene; The influence of PPO-A1 gene on polyphenol oxidase activity was more significant than PPO-D1 gene. The high protein content of varieties mostly containing hardness genes and 1/2*/5 + 10 subunit combinations. Based on the present study, we found that the quality gene distribution of Chinese and CIMMYT varieties was quite different, for instance, the high-quality HMW-GS subunits of Chinese varieties were lower than CIMMYT lines. It will be much useful for Chinese wheat breeders to develop good quality wheat variety by crossing with 3 good strong gluten CIMMYT wheat lines by molecular marker-assisted selection.

Keywords: KASP marker; molecular markers; quality property; quality related genes; wheat.

Grants and funding

This work was supported by development and application of new wheat varieties with good quality and high yield for the Middle and Lower Reaches of the Yangtze River (NK2022060601), Key Research and Development Program of Hubei Province (2022BBA0035), HZAU-AGIS Co-operation Fund (SZYJY2022035), Xinjiang Bingtuan Science and Technology Program (2019AB021, 2016AC027, 2022DB013, and 2022CA006), The National Natural Science Foundation of China (U1178306 and 31560397).