Case report: Effect of Hb E heterozygosity on HbA1c value by the Tosoh HLC-723G11

Front Public Health. 2023 Aug 3:11:1217662. doi: 10.3389/fpubh.2023.1217662. eCollection 2023.

Abstract

Objective: We report the effect of Hb E heterozygosity on HbA1c value by the Tosoh HLC-723G11.

Case report: A 45 years-old Chinese woman presented with an abnormally low HbA1c level of 3.7% (3.9%-6.1%) in a health examination. Fasting blood glucose was normal. Blood routine examination and serum bilirubin were in the normal range. HbA1c was determined by Tosoh HLC-723G11. There was an abnormal peak between A1c and A0 on the chromatogram. Hemoglobin electrophoresis indicated that the Hb E zone accounted for 25.1%. The β-thalassemia-related genes (mutant type) were βE M/N, and the related gene CD26 (A > G) was mutated. OGTT indicated prediabetes.

Conclusion: Hb E heterozygosity may reduce HbA1c value with abnormal chromatograms, as determined by a Tosoh HLC G11 analyzer. The Tosoh HLC G11 analyzer can well identify Hb E variation. In this case, further blood glucose-related tests should be performed to avoid missed diagnoses. However, a large sample size is needed to confirm this conclusion.

Keywords: Hb E; HbA1c; Tosoh HLC-723G11; heterozygosity; high-pressure liquid chromatography.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asian People
  • Blood Glucose*
  • Female
  • Glycated Hemoglobin / genetics
  • Humans
  • Middle Aged
  • Prediabetic State*
  • Reference Values

Substances

  • Blood Glucose
  • Glycated Hemoglobin