Flow Cytometry Analysis of IL-1 Receptors and Toll-Like Receptors on Platelets and Platelet-Derived Extracellular Vesicles

Achille Anselmo; Daniela Boselli

doi:10.1007/978-1-0716-3366-3_6

Flow Cytometry Analysis of IL-1 Receptors and Toll-Like Receptors on Platelets and Platelet-Derived Extracellular Vesicles

Methods Mol Biol. 2023:2700:117-137. doi: 10.1007/978-1-0716-3366-3_6.

Authors

Achille Anselmo¹, Daniela Boselli²

Affiliations

¹ Flow Cytometry Resource, Advanced Cytometry Technical Applications Laboratory, San Raffaele Scientific Institute, Milan, Italy. anselmo.achille@hsr.it.
² Flow Cytometry Resource, Advanced Cytometry Technical Applications Laboratory, San Raffaele Scientific Institute, Milan, Italy.

PMID: 37603177
DOI: 10.1007/978-1-0716-3366-3_6

Abstract

Flow cytometry is largely used for the immunophenotyping and quantification of several cell types or related components including platelets and extracellular vesicles. Platelets and platelet-derived extracellular vesicles (PEVs) are receiving increased interest in inflammatory diseases including sepsis. Thus, in this chapter, we will describe protocols for the flow cytometry analysis of platelets, platelet/neutrophils hetero aggregates, and PEVs mainly focusing on the evaluation of the surface expression of some IL-1 receptor (ILR) and Toll-like receptor (TLR) family members.

Keywords: Antibody; Extracellular vesicles; Flow cytometry; Immunophenotyping; Inflammation; Instrument setting; Platelets.

MeSH terms

Blood Platelets*
Extracellular Vesicles*
Flow Cytometry
Receptors, Interleukin-1
Toll-Like Receptors

Substances

Receptors, Interleukin-1
Toll-Like Receptors