Effect of cigarette-smoke condensate and norharman on the induction of SCEs by direct and indirect mutagens in CHO cells

Mutat Res. 1986 Oct;172(1):61-7. doi: 10.1016/0165-1218(86)90107-2.

Abstract

Cigarette-smoke condensate and norharman were investigated either alone or in combination with a number of direct or indirect mutagens for the induction of sister-chromatid exchanges (SCEs) in Chinese hamster ovary (CHO) cells. Cigarette-smoke condensate and norharman induced SCEs in these cells, only in the presence of a metabolic activation system. The number of SCEs induced by the direct-acting mutagens mitomycin C and N-methyl-N'-nitro-N-nitrosoguanidine was decreased in the presence of cigarette-smoke condensate or norharman. However, cigarette-smoke condensate and norharman showed synergistic effects in combination with the indirect mutagens 2-acetylaminofluorene, 2-aminofluorene, N-hydroxy-acetylaminofluorene, 2-aminoanthracene and benzo[a]pyrene. No synergism was observed when CHO cells were treated simultaneously with cigarette-smoke condensate or norharman and the indirect mutagen cyclophosphamide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / toxicity*
  • Animals
  • Carbolines
  • Cell Line
  • Cricetinae
  • Drug Synergism
  • Harmine / analogs & derivatives
  • Harmine / toxicity*
  • Mutagenicity Tests
  • Mutagens*
  • Plants, Toxic
  • Sister Chromatid Exchange / drug effects*
  • Smoke*
  • Tobacco

Substances

  • Alkaloids
  • Carbolines
  • Mutagens
  • Smoke
  • Harmine
  • norharman