Systems biology based miRNA-mRNA expression pattern analysis of Emodin in breast cancer cell lines

Elif Sakalli-Tecim; Bala Gur-Dedeoglu; N Tulin Guray

doi:10.1016/j.prp.2023.154780

Systems biology based miRNA-mRNA expression pattern analysis of Emodin in breast cancer cell lines

Pathol Res Pract. 2023 Sep:249:154780. doi: 10.1016/j.prp.2023.154780. Epub 2023 Aug 22.

Authors

Elif Sakalli-Tecim¹, Bala Gur-Dedeoglu², N Tulin Guray³

Affiliations

¹ Department of Biotechnology, Middle East Technical University, Ankara, Turkiye.
² Biotechnology Institute, Ankara University, Ankara, Turkiye.
³ Department of Biotechnology, Middle East Technical University, Ankara, Turkiye; Department of Biological Sciences, Middle East Technical University, Ankara, Turkiye. Electronic address: guray@metu.edu.tr.

PMID: 37633004
DOI: 10.1016/j.prp.2023.154780

Abstract

Breast cancer has been among the most prominent cancers with high mortality. Currently most of the offered therapeutics are toxic; hence, less toxic therapeutic intervention is required. Here, we studied the molecular mechanisms of the effect of a phytoestrogen Emodin on estrogen receptor positive MCF-7 and negative MDA-MB-231 cells by carrying out a comprehensive network assessment. Differentially expressed microRNAs along with their previously identified differentially expressed mRNAs were analyzed through microarrays by using integrative systems biology approach. For each cell line miRNA-target gene networks were built, gene ontology and pathway enrichment analyses were performed, enrichment maps were constructed and the potential key genes, miRNAs and miRNA-gene interactions were studied.

Keywords: Breast cancer; Emodin; Estrogen receptor; Phytoestrogen; Systems biology; miRNA.

MeSH terms

Breast Neoplasms* / drug therapy
Breast Neoplasms* / genetics
Emodin* / pharmacology
Female
Humans
MCF-7 Cells
MicroRNAs* / genetics
RNA, Messenger
Systems Biology

Substances

MicroRNAs
Emodin
RNA, Messenger