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Found 1 result for 2023RC046/the Zhejiang Provincial Science and Technology Plan Project of China[Grants and Funding]
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. 2023 Aug 30;24(1):506.
doi: 10.1186/s12864-023-09579-9.

Complete-genome sequencing and comparative genomic characterization of blaNDM-5 carrying Citrobacter freundii isolates from a patient with multiple infections

Jianzhong Ye #  1 , Lulu Jin #  2 , Yaling Li #  3 , Hao Xu #  4   5 , Yishuai Lin  1 , Tieli Zhou  1 , Beiwen Zheng  6 , Maofeng Wang  7 , Zhongyong Wang  8
Affiliations

Complete-genome sequencing and comparative genomic characterization of blaNDM-5 carrying Citrobacter freundii isolates from a patient with multiple infections

Jianzhong Ye et al. BMC Genomics. .

Abstract

Background: The emergence and wide spread of carbapenemase-producing Enterobacteriaceae (CPE) poses a growing threat to global public health. However, clinically derived carbapenemase-producing Citrobacter causing multiple infections has rarely been investigated. Here we first report the isolation and comparative genomics of two blaNDM-5 carrying Citrobacter freundii (C. freundii) isolates from a patient with bloodstream and urinary tract infections.

Results: Antimicrobial susceptibility testing showed that both blaNDM-5 carrying C. freundii isolates were multidrug-resistant. Positive modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) results suggested metallo-carbapenemase production. PCR and sequencing confirmed that both metallo-carbapenemase producers were blaNDM-5 positive. Genotyping and comparative genomics analyses revealed that both isolates exhibited a high level of genetic similarity. Plasmid analysis confirmed that the blaNDM-5 resistance gene is located on IncX3 plasmid with a length of 46,161 bp, and could successfully be transferred to the recipient Escherichia coli EC600 strain. A conserved structure sequence (ISAba125-IS5-blaNDM-5-trpF-IS26-umuD-ISKox3) was found in the upstream and downstream of the blaNDM-5 gene.

Conclusions: The data presented in this study showed that the conjugative blaNDM-5 plasmid possesses a certain ability to horizontal transfer. The dissemination of NDM-5-producing C. freundii isolates should be of close concern in future clinical surveillance. To our knowledge, this is the first study to characterize C. freundii strains carrying the blaNDM-5 gene from one single patient with multiple infections.

Keywords: Antimicrobial resistance; Horizontal gene transfer; IncX plasmid; Plasmid conjugation.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
The core-genome phylogenetic tree generated by kSNP. Blue color of the right panel indicates positive antibiotic resistance genes of the corresponding strains
Fig. 2
Fig. 2
blaNDM-5 gene location analysis. A S1-PFGE of both blaNDM-5 carrying C. freundii isolates DY2007 and DY2010. Salmonella enterica serotype H9812 was used as molecular marker. B Corresponding Southern blotting analysis using blaNDM-5-specific probe. A and B was cropped from different gels. Full-length blots/gels are presented in Supplementary Fig. 2
Fig. 3
Fig. 3
Genomic analyses of pNDM-5 plasmid. A Comparison of the pNDM-5 plasmid sequence identified in isolates DY2007 and DY2010 with Escherichia coli strain WCHEC020031 plasmid pNDM5_020031 (GenBank accession number: CP033399.1), Klebsiella pneumoniae strain 19,110,124 plasmid p19110124-3 (GenBank accession number: CP064177.1), and Escherichia coli strain L53 plasmid pL53-4 (GenBank accession number: CP034737.1). The figure was plotted using BRIG, and the blaNDM-5 gene was highlighted in red. B Genetic environment of blaNDM-5 on pNDM-5 and related plasmids. Open reading frames were indicated as arrows. Shared areas with highly similar sequences were drawn by lake green. Conjugal transfer associated genes were shown by brown; blaNDM-5 gene were indicated by red arrows; functional protein coding genes were colored by green; other antibiotic resistance genes were colored by blue
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