Melatonin attenuates sepsis-induced acute kidney injury by promoting mitophagy through SIRT3-mediated TFAM deacetylation

Zhiya Deng; Man He; Hongbin Hu; Wenqian Zhang; Yaoyuan Zhang; Yue Ge; Tongtong Ma; Jie Wu; Lulan Li; Maomao Sun; Sheng An; Jiaxin Li; Qiaobing Huang; Shenhai Gong; Jiaxing Zhang; Zhongqing Chen; Zhenhua Zeng

doi:10.1080/15548627.2023.2252265

Melatonin attenuates sepsis-induced acute kidney injury by promoting mitophagy through SIRT3-mediated TFAM deacetylation

Autophagy. 2024 Jan;20(1):151-165. doi: 10.1080/15548627.2023.2252265. Epub 2023 Aug 31.

Authors

Zhiya Deng^{1

2}, Man He^{1

2}, Hongbin Hu¹, Wenqian Zhang^{1

2}, Yaoyuan Zhang¹, Yue Ge^{1

3}, Tongtong Ma¹, Jie Wu¹, Lulan Li¹, Maomao Sun^{1

2}, Sheng An^{1

2}, Jiaxin Li¹, Qiaobing Huang^{1

2}, Shenhai Gong^{1

4}, Jiaxing Zhang^{1

5}, Zhongqing Chen¹, Zhenhua Zeng^{1

2}

Affiliations

¹ Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
² Guangdong Provincial Key Laboratory of Cardiac Function and Microcirculation, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.
³ School of Nursing, Southern Medical University, Guangzhou, Guangdong, China.
⁴ School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
⁵ The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, China.

Abstract

AKI: acute kidney injury; ATP: adenosine triphosphate; BUN: blood urea nitrogen; CLP: cecal ligation and puncture; eGFR: estimated glomerular filtration rate; H&E: hematoxylin and eosin staining; LCN2/NGAL: lipocalin 2; LPS: lipopolysaccharide; LTL: lotus tetragonolobus lectin; mKeima: mitochondria-targeted Keima; mtDNA: mitochondrial DNA; PAS: periodic acid - Schiff staining; RTECs: renal tubular epithelial cells; SAKI: sepsis-induced acute kidney injury; Scr: serum creatinine; SIRT3: sirtuin 3; TFAM: transcription factor A, mitochondrial; TMRE: tetramethylrhodamine.

Keywords: Acetylation; acute kidney injury; autophagy; critical care; post-translational modification; sepsis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acute Kidney Injury*
Autophagy
DNA, Mitochondrial
DNA-Binding Proteins
Humans
Kidney
Lipopolysaccharides
Melatonin*
Mitochondrial Proteins
Mitophagy
Sepsis* / complications
Sirtuin 3*
Transcription Factors

Substances

Sirtuin 3
Melatonin
Lipopolysaccharides
DNA, Mitochondrial
TFAM protein, human
DNA-Binding Proteins
Transcription Factors
Mitochondrial Proteins
SIRT3 protein, human