In vivo biological analysis of cold plasma on allergenicity reduction of tropomyosin in shrimp

Jun-Hu Cheng; Jilin Li; Da-Wen Sun

doi:10.1016/j.foodchem.2023.137210

In vivo biological analysis of cold plasma on allergenicity reduction of tropomyosin in shrimp

Food Chem. 2024 Jan 30:432:137210. doi: 10.1016/j.foodchem.2023.137210. Epub 2023 Aug 19.

Authors

Jun-Hu Cheng¹, Jilin Li¹, Da-Wen Sun²

Affiliations

¹ School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China.
² School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Food Refrigeration and Computerized Food Technology (FRCFT), Agriculture and Food Science Centre, University College Dublin, National University of Ireland, Belfield, Dublin 4, Ireland. Electronic address: dawen.sun@ucd.ie.

PMID: 37659333
DOI: 10.1016/j.foodchem.2023.137210

Abstract

In vivo biological regulations of the allergenicity of tropomyosin (TM) treated by cold plasma (CP) were investigated by in vivo mouse model. The sensitization models of Balb/c mice were successfully established. CP treatment reduced the allergic symptoms of mice and regulated the Th1/Th2 balance to prevent allergy by activating Treg cells, which was deduced by serum and cytokines analysis. For intestinal flora analysis, allergy occurrence was accompanied by the decreased species abundance and the increased species diversity of intestinal flora. The significant species composition difference between the TM group and the PBS group showed a possible connection between bacterial diversity and allergy. Furthermore, Firmicutes, Bacteroidetes, Parabacteroides, Alloprevotella, Bacteroides, and Lachnospiraceae could relate to allergy occurrence. Intestinal section analysis suggested that allergy occurrence was accompanied by the damaged intestinal structure, and CP treatment could relieve the damage caused by an allergy.

Keywords: Allergenicity; Cold plasma; Intestine flora; Mouse model; Tropomyosin.

MeSH terms

Allergens
Animals
Bacteroidetes
Crustacea
Hypersensitivity*
Mice
Mice, Inbred BALB C
Plasma Gases*
Tropomyosin / genetics

Substances

Allergens
Plasma Gases
Tropomyosin