dCas9-3xSRDX-mediated transcriptional repression in sugarcane

Plant Cell Rep. 2023 Nov;42(11):1837-1840. doi: 10.1007/s00299-023-03062-1. Epub 2023 Sep 4.

Isidre Hooghvorst^{1

2}, Fredy Altpeter^{3

4}

¹ Agronomy Department, Plant Molecular and Cellular Biology Program, Genetics Institute, University of Florida, IFAS, Gainesville, FL, USA.
² DOE, Center for Advanced Bioenergy and Bioproducts Innovation, Gainesville, FL, USA.
³ Agronomy Department, Plant Molecular and Cellular Biology Program, Genetics Institute, University of Florida, IFAS, Gainesville, FL, USA. altpeter@ufl.edu.
⁴ DOE, Center for Advanced Bioenergy and Bioproducts Innovation, Gainesville, FL, USA. altpeter@ufl.edu.

Abstract

Targeting dCas9 fused with the 3xSRDX effector to the 5'UTR leads to strong repression of magnesium chelatase in highly polyploid sugarcane.