Developmental Toxicity Assessment Using Zebrafish-Based High-Throughput Screening

Methods Mol Biol. 2024:2707:71-82. doi: 10.1007/978-1-0716-3401-1_4.


Zebrafish-based high-throughput screening has been extensively used to study toxicological profiles of individual chemicals and mixtures, identify novel toxicants, and study modes of action to prioritize chemicals for further testing and policy decisions. Within this chapter, we describe a protocol for automated zebrafish developmental high-throughput screening in our laboratory, with emphasis on exposure setups, morphological and behavioral readouts, and quality control.

Keywords: Automation; Behavior; Chemicals; Development; Embryos; High-throughput screening; Morphology; Zebrafish.

MeSH terms

  • Animals
  • Hazardous Substances
  • High-Throughput Screening Assays*
  • Laboratories
  • Policy
  • Zebrafish*


  • Hazardous Substances