Effects of nitrogen addition on species composition and diversity of early spring herbs in a Korean pine plantation

Guanghui Yang; Mengmeng Zhang; Guangze Jin

doi:10.1002/ece3.10498

Effects of nitrogen addition on species composition and diversity of early spring herbs in a Korean pine plantation

Ecol Evol. 2023 Sep 5;13(9):e10498. doi: 10.1002/ece3.10498. eCollection 2023 Sep.

Authors

Guanghui Yang¹, Mengmeng Zhang^{1

2}, Guangze Jin^{1

3

4}

Affiliations

¹ Center for Ecological Research Northeast Forestry University Harbin China.
² College of Life Science Heilongjiang University Harbin China.
³ Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education Northeast Forestry University Harbin China.
⁴ Northeast Asia Biodiversity Research Center Northeast Forestry University Harbin China.

Abstract

Under the background of global nitrogen deposition, temperate forest ecosystems are suffering increasing threats, and species diversity is gradually decreasing. In this study, nitrogen addition experiments were conducted on Korean pine (Pinus koraiensis) plantations in Northeast China to explore the effect of long-term nitrogen addition on herb species diversity to test the following hypothesis: long-term nitrogen addition further reduced plant species diversity by affecting plant growth, which may be due to soil acidification caused by excessive nitrogen addition. Experimental nitrogen addition was conducted from 2014 to 2021, and the nitrogen treatment levels were as follows: N0 (control treatment, 0/(kg N ha^-1 year^-1)), N20 (low nitrogen treatment, 20/(kg N ha^-1 year^-1)), N40 (medium nitrogen treatment, 40/(kg N ha^-1 year^-1)) and N80 (high nitrogen treatment, 80/(kg N ha^-1 year^-1)). A herb community survey was conducted in the region from 2015 to 2021. The results showed that long-term nitrogen addition decreased soil pH, changed the species and composition of herbaceous plants, and decreased the species diversity of understory herbaceous plants. With the increase in nitrogen application years, middle- and high-nitrogen treatments significantly reduced the diversity of early-spring flowering herbs and early-spring foliating herbs, and their diversity decreased with the decrease in soil pH, indicating that soil acidification caused by long-term nitrogen addition may lead to the decrease of plant diversity. However, for early-spring growing herbs, adequate nitrogen addition may promote their growth. Our results show that plants have evolved different life-history strategies based on their adaptation mechanisms to the environment, and different life-history strategies have different responses to long-term nitrogen addition. However, for most plants, long-term nitrogen application will have a negative impact on the growth and diversity of herbs in temperate forests.

Keywords: Korean pine plantation; early spring herbs; nitrogen addition; soil property; species diversity; understory light.