A sensitive hemolytic assay for mouse C3

J Immunol Methods. 1979;26(3):283-90. doi: 10.1016/0022-1759(79)90253-9.


We have developed a sensitive hemolytic assay for mouse C3, utilizing readily available guinea pig complement components and standard techniques, which allows C3 to be detected in mouse serum diluted many thousand-fold. Mouse serum also contains a potent, relatively heat-stable C3-inactivator system, which is largely blocked by Suramin.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Complement C3 / isolation & purification*
  • Elapid Venoms / pharmacology
  • Female
  • Hemolytic Plaque Technique*
  • Hot Temperature
  • Mice
  • Mice, Inbred Strains / blood*
  • Osmolar Concentration
  • Suramin / pharmacology
  • Time Factors


  • Complement C3
  • Elapid Venoms
  • Suramin