Abstract
We have developed a sensitive hemolytic assay for mouse C3, utilizing readily available guinea pig complement components and standard techniques, which allows C3 to be detected in mouse serum diluted many thousand-fold. Mouse serum also contains a potent, relatively heat-stable C3-inactivator system, which is largely blocked by Suramin.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Complement C3 / isolation & purification*
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Elapid Venoms / pharmacology
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Female
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Hemolytic Plaque Technique*
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Hot Temperature
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Mice
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Mice, Inbred Strains / blood*
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Osmolar Concentration
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Suramin / pharmacology
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Time Factors
Substances
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Complement C3
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Elapid Venoms
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Suramin