Use of Atomic Force Microscopy in UVB-Induced Chromosome Damage Provides Important Bioinformation for Cell Damage Assessment

Bowei Wang; Jianjun Dong; Fan Yang; Tuoyu Ju; Jiani Li; Junxi Wang; Ying Wang; M James C Crabbe; Yanling Tian; Zuobin Wang

doi:10.1021/acs.langmuir.3c01644

Use of Atomic Force Microscopy in UVB-Induced Chromosome Damage Provides Important Bioinformation for Cell Damage Assessment

Langmuir. 2023 Sep 19;39(37):13212-13221. doi: 10.1021/acs.langmuir.3c01644. Epub 2023 Sep 8.

Authors

Bowei Wang^{1

2}, Jianjun Dong^{1

2}, Fan Yang^{1

2}, Tuoyu Ju^{1

2}, Jiani Li^{1

2}, Junxi Wang^{1

2}, Ying Wang^{1

2}, M James C Crabbe^{3

4}, Yanling Tian⁵, Zuobin Wang^{1

2

4}

Affiliations

¹ International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China.
² Centre for Opto/Bio-Nano Measurement and Manufacturing, Zhongshan Institute of Changchun University of Science and Technology, Zhongshan 528437, China.
³ Wolfson College, University of Oxford, Oxford OX2 6UD, U.K.
⁴ Institute of Biomedical and Environmental Science & Technology, and Institute for Research in Applicable Computing, University of Bedfordshire, Luton LU1 3JU, U.K.
⁵ School of Engineering, University of Warwick, Coventry CV4 7AL, U.K.

PMID: 37681704
DOI: 10.1021/acs.langmuir.3c01644

Abstract

The chromosomal structure derived from UVB-stimulated HaCaT cells was detected by atomic force microscopy (AFM) to evaluate the effect of UVB irradiation. The results showed that the higher the UVB irradiation dose, the more the cells that had chromosome aberration. At the same time, different representative types of chromosome structural aberrations were investigated. We also revealed damage to both DNA and cells under the corresponding irradiation doses. It was found that the degree of DNA damage was directly proportional to the irradiation dose. The mechanical properties of cells were also changed after UVB irradiation, suggesting that cells experienced a series of chain reactions from inside to outside after irradiation. The high-resolution imaging of chromosome structures by AFM after UVB irradiation enables us to relate the damage between chromosomes, DNA, and cells caused by UVB irradiation and provides specific information on genetic effects.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes
DNA Damage*
Microscopy, Atomic Force
Ultraviolet Rays* / adverse effects