Ethoxyresorufin O-deethylation by human liver microsomes

Br J Clin Pharmacol. 1986 Sep;22(3):263-8. doi: 10.1111/j.1365-2125.1986.tb02885.x.


As a substrate for human liver microsomes, ethoxyresorufin appears to be metabolised by a group of cytochrome P450 isoenzymes which are inducible by cigarette smoking. Kinetic studies in microsomes from four human livers indicate that only one enzyme component is involved over the full substrate range. Ethoxyresorufin O-deethylation activity at both high and low substrate concentrations correlated with the high affinity component of ethoxycoumarin O-deethylation and of diphenyloxazole metabolism.

MeSH terms

  • Adult
  • Aged
  • Coumarins / metabolism
  • Female
  • Fluorometry
  • Humans
  • Kinetics
  • Male
  • Microsomes, Liver / enzymology
  • Microsomes, Liver / metabolism*
  • Middle Aged
  • Oxazines / metabolism*
  • Oxazoles / metabolism
  • Smoking


  • Coumarins
  • Oxazines
  • Oxazoles
  • 7-ethoxycoumarin
  • ethoxyresorufin
  • 2,5-diphenyloxazole