Evaluating the Performance of the Astral Mass Analyzer for Quantitative Proteomics Using Data-Independent Acquisition

J Proteome Res. 2023 Oct 6;22(10):3290-3300. doi: 10.1021/acs.jproteome.3c00357. Epub 2023 Sep 8.


We evaluate the quantitative performance of the newly released Asymmetric Track Lossless (Astral) analyzer. Using data-independent acquisition, the Thermo Scientific Orbitrap Astral mass spectrometer quantifies 5 times more peptides per unit time than state-of-the-art Thermo Scientific Orbitrap mass spectrometers, which have long been the gold standard for high-resolution quantitative proteomics. Our results demonstrate that the Orbitrap Astral mass spectrometer can produce high-quality quantitative measurements across a wide dynamic range. We also use a newly developed extracellular vesicle enrichment protocol to reach new depths of coverage in the plasma proteome, quantifying over 5000 plasma proteins in a 60 min gradient with the Orbitrap Astral mass spectrometer.

Keywords: data-independent acquisition; high-resolution mass spectrometry; plasma; quantitative proteomics.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Blood Proteins
  • Mass Spectrometry / methods
  • Peptides*
  • Proteome / metabolism
  • Proteomics* / methods


  • Peptides
  • Proteome
  • Blood Proteins