A surface multiple imprinting layers membrane with well-oriented recognition sites for selective separation of chlorogenic acid from Ficus carica L

Zidan Luo; Mengfei Tian; Naveed Ahmad; Yuxin Xie; Chunguo Xu; Jie Liu; Chunjian Zhao; Chunying Li

doi:10.1016/j.foodchem.2023.137347

A surface multiple imprinting layers membrane with well-oriented recognition sites for selective separation of chlorogenic acid from Ficus carica L

Food Chem. 2024 Feb 1:433:137347. doi: 10.1016/j.foodchem.2023.137347. Epub 2023 Sep 3.

Authors

Zidan Luo¹, Mengfei Tian¹, Naveed Ahmad², Yuxin Xie¹, Chunguo Xu³, Jie Liu⁴, Chunjian Zhao⁵, Chunying Li⁶

Affiliations

¹ College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Ministry of Education, Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances, Northeast Forestry University, Harbin 150040, China.
² Department of Chemistry, Division of Science and Technology, University of Education, Lahore, Pakistan.
³ Dasuhe Forest Farm, Qingyuan, Liaoning 113312, China.
⁴ Hisun Pharmaceutical (Hangzhou) Co., Ltd., No. 1, Road, Xukou Town, Fuyang Distrist, Hangzhou 311404, China.
⁵ College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Ministry of Education, Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances, Northeast Forestry University, Harbin 150040, China. Electronic address: zcj@nefu.edu.cn.
⁶ College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Ministry of Education, Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances, Northeast Forestry University, Harbin 150040, China. Electronic address: lcy@nefu.edu.cn.

PMID: 37683463
DOI: 10.1016/j.foodchem.2023.137347

Abstract

Chlorogenic acid (CGA), known as an important natural antioxidative compound in Ficus carica L, has valuable application prospects on health food, functional food, nutrition and dietary formulations. In this study, a surface multiple imprinting layers membrane (SMILM) was developed and applied to separate CGA from F. carica. Two different imprinting layers were integrated onto the membrane surface in sequence. The first imprinting layer was formed by dopamine polymerization and the second imprinting layer was fabricated by atom transfer radical polymerization (ATRP) and boronate affinity. The prepared SMILM with well-oriented multiple recognition sites exhibited high adsorption capacity (52.08 mg·g^-1 in 60 min) for CGA and specific selectivity with imprinting factor (IF) of 3.06. Furthermore, the recognition mechanism of SMILM was clarified by molecular simulation and NMR. The SMILM was successfully applied to separate CGA from the fruits, peels and leaves of F. carica with recoveries of 90.22, 83.31 and 84.95 %, respectively.

Keywords: Chlorogenic acid; Ficus carica L; Molecularly imprinted membrane; Multiple imprinting layers; Oriented recognition.

MeSH terms

Adsorption
Antioxidants
Chlorogenic Acid / chemistry
Ficus*
Molecular Imprinting*
Polymers / chemistry

Substances

Chlorogenic Acid
Polymers
Antioxidants