The blood group U antigen is not located on glycophorin B

Biochim Biophys Acta. 1986 Nov 19;884(2):337-43. doi: 10.1016/0304-4165(86)90182-0.


Erythrocytes from twelve individuals with the S-s-U- phenotype and from ten with the S-S-U+ phenotype were analyzed and compared to control cells with S+/s+U+ determinants. No red cell abnormality was detected in S-s-U+ or S-s-U- carriers. Sialic acid content was similar (P greater than 0.05) for S-s-U+ and S-s-U- erythrocytes (74.6 +/- 7.14 and 71.4 +/- 8.53 nmol/10(9) red blood cells, respectively) but significantly less (P less than 0.001) than controls with 89.5 +/- 11.4 nmol/10(9) red blood cells, n = 16. Fluorographs of SDS-polyacrylamide gels showed no glycophorin B in membranes from S-s-U+ and S-s-U- erythrocytes labeled with NaB3H4. Glycophorins were extracted from red cell membranes in chloroform/methanol, labeled with 125I and analyzed by SDS-polyacrylamide gel electrophoresis. Periodic acid Schiff stain and autoradiographs of these gels also showed absence of glycophorin B in both S-s-U+ and S-s-U- cells. These findings suggested that the U antigen is not located on glycophorin B. This hypothesis was tested by determining blood group antigenicity on red cell membranes and on extracted sialoglycoproteins by the hemagglutination inhibition technique. Although U and S/s activities were detected in control red cell membranes, extracted glycoproteins demonstrated S/s activity but no U activity. Together the data indicate that both S-s-U+ and S-s-U- erythrocytes lack glycophorin B and that the U antigen is not located on glycophorin B. This deletion does not seem to affect the structure-function of the red cell.

MeSH terms

  • Electrophoresis, Polyacrylamide Gel
  • Erythrocyte Membrane / immunology
  • Erythrocytes / immunology*
  • Glycophorins / analysis*
  • Glycoproteins / blood
  • Glycoproteins / immunology
  • Humans
  • Isoantigens / analysis*
  • MNSs Blood-Group System / immunology*
  • Membrane Proteins / blood
  • N-Acetylneuraminic Acid
  • Phenotype
  • Sialic Acids / blood
  • Sialoglycoproteins / analysis*


  • Glycophorins
  • Glycoproteins
  • Isoantigens
  • MNSs Blood-Group System
  • Membrane Proteins
  • Sialic Acids
  • Sialoglycoproteins
  • N-Acetylneuraminic Acid