The interaction among Kluyveromyces marxianus G-Y4, Lacticaseibacillus paracasei GL1, and Lactobacillus helveticus SNA12 and signaling molecule AI-2 participate in the formation of biofilm

Xiaomeng Wang; Kai Ma; Changliang Zhang; Feng Ji; Lili Chen; Xueliang Zhang; Ghahvechi Chaeipeima Mahsa; Elham Azarpazhooh; Marjan Ajami; Xin Rui; Wei Li

doi:10.1016/j.fm.2023.104369

The interaction among Kluyveromyces marxianus G-Y4, Lacticaseibacillus paracasei GL1, and Lactobacillus helveticus SNA12 and signaling molecule AI-2 participate in the formation of biofilm

Food Microbiol. 2023 Dec:116:104369. doi: 10.1016/j.fm.2023.104369. Epub 2023 Aug 25.

Authors

Xiaomeng Wang¹, Kai Ma², Changliang Zhang², Feng Ji², Lili Chen³, Xueliang Zhang⁴, Ghahvechi Chaeipeima Mahsa⁴, Elham Azarpazhooh⁵, Marjan Ajami⁶, Xin Rui⁴, Wei Li⁷

Affiliations

¹ Sanya Institute of Nanjing Agricultural University, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China; College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong, 266404, PR China.
² Jiangsu New-Bio Biotechnology Co., Ltd., Jiangyin, Jiangsu, 214400, PR China; Jiangsu Biodep Biotechnology Co., Ltd., Jiangyin, Jiangsu, 214400, PR China.
³ Xinyi Ziyuantian Biotechnology Development Co., Ltd, Xinyi, Jiangsu, 221400, PR China.
⁴ Sanya Institute of Nanjing Agricultural University, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China.
⁵ Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Iran.
⁶ National Nutrition and Food Technology Research Institute, School of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Science, Tehran, Iran.
⁷ Sanya Institute of Nanjing Agricultural University, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China. Electronic address: lw1981@njau.edu.cn.

PMID: 37689420
DOI: 10.1016/j.fm.2023.104369

Abstract

In this study, two strains of lactic acid bacteria (Lacticaseibacillus paracasei GL1 and Lactobacillus helveticus SNA12) and one yeast strain of Kluyveromyces marxianus G-Y4 (G-Y4) isolated from Tibetan kefir grains were co-cultured. It was found that the addition of G-Y4 could not only promote the growth of lactic acid bacteria, but also increase the release of metabolites (lactic acid, ethanol, and amino nitrogen). Furthermore, the addition of live cells and cell-free fermentation supernatant (CFS) of G-Y4 could increase the ability of biofilm formation. Morever, the surface characteristics results showed that the addition of G-Y4 live cells could enhance the aggregation ability and hydrophobicity of LAB. Meanwhile, adding live cells and CFS of G-Y4 could promote the release of signaling molecule AI-2 and enhance the expression of the LuxS gene related to biofilm formation. In addition, Fourier-transform infrared spectroscopy and chemical composition analysis were used to investigate the composition of the biofilm, and the results indicated that the biofilm was mainly composed of a small amount of protein but it was rich in polysaccharides including glucose, galactose, and mannose with different ratios. Finally, the formation of biofilm could delay the decline of the number of viable bacteria in storage fermented milk.

Keywords: Biofilm formation; Kluyveromyces marxianus; Lactic acid bacteria; Polysaccharide; Signaling molecule AI-2.

MeSH terms

Biofilms
Kluyveromyces* / genetics
Lacticaseibacillus
Lacticaseibacillus paracasei*
Lactobacillus helveticus* / genetics

Supplementary concepts

Kluyveromyces marxianus