Dynamic plasmid copy number control for synthetic biology

Gege Wang; Qi Wang; Qingsheng Qi; Qian Wang

doi:10.1016/j.tibtech.2023.08.004

Dynamic plasmid copy number control for synthetic biology

Trends Biotechnol. 2024 Feb;42(2):147-150. doi: 10.1016/j.tibtech.2023.08.004. Epub 2023 Sep 7.

Authors

Gege Wang¹, Qi Wang¹, Qingsheng Qi¹, Qian Wang²

Affiliations

¹ National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, PR China.
² National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, PR China. Electronic address: qiqi20011983@gmail.com.

PMID: 37689527
DOI: 10.1016/j.tibtech.2023.08.004

Abstract

Plasmids that replicate independently from chromosomes are valuable genetic tools for biological research. Dynamic control of plasmid copy number facilitates flexible regulation of the gene of interest or the genetic circuit installed in the plasmid. This useful strategy is being integrated into synthetic biology for metabolic reprogramming and biosensing applications.

Keywords: biosensor; dynamic control; metabolic reprogramming; plasmid copy number; plasmid replication regulation machinery; synthetic biology.

MeSH terms

DNA Copy Number Variations*
Plasmids / genetics
Synthetic Biology*