Preparation, Characterization, and Molecular Dynamic Simulation of Novel Coenzyme Q10 Loaded Nanostructured Lipid Carriers

Hoda Atapour-Mashhad; Mojgan Nejabat; Farzin Hadizadeh; Afsaneh Hoseinsalari; Shiva Golmohammadzadeh

doi:10.2174/1381612829666230911105913

Preparation, Characterization, and Molecular Dynamic Simulation of Novel Coenzyme Q10 Loaded Nanostructured Lipid Carriers

Curr Pharm Des. 2023;29(27):2177-2190. doi: 10.2174/1381612829666230911105913.

Authors

Hoda Atapour-Mashhad^{1

2}, Mojgan Nejabat³, Farzin Hadizadeh⁴, Afsaneh Hoseinsalari⁵, Shiva Golmohammadzadeh^{1

2}

Affiliations

¹ Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
² Department of Pharmaceutics, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
³ Department of Medicinal Chemistry, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
⁴ Biotechnology Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, Mashhad, Iran.
⁵ Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

PMID: 37694784
DOI: 10.2174/1381612829666230911105913

Abstract

Background: Research proved that coenzyme Q10-loaded NLC effectively removes skin wrinkles, therefore, such a formulation with good characteristics is still the research goal.

Objective: This study investigated the effect of solid lipids and surfactant type on the physical characteristics of Q10-NLC. We aimed to achieve the optimum formulation for producing NLC with long-term stability and high Entrapment efficiency (E.E.) %. We compared the experimental results with the output of the Molecular dynamic (M.D.) simulations.

Methods: To develop Q10-NLC, various solid lipids, MCT oil, and surfactants were employed. The formulations were prepared by high-shear homogenization and ultrasound methods. Stability studies were carried out 1,3, and 6 months at 4, 25, and 40°C. The optimized NLC formulations were characterized by photon correlation spectroscopy (PCS), Transmission electron microscopy (TEM), Differential scanning calorimetry (DSC), and Fourier transform infrared (FT-IR). E.E. % was determined by HPLC analysis. Atomistic M.D. simulations of two model systems were performed to gain insights into the self-assembled process of co-Q10 with other formulation components.

Results: Statistical analysis (Two-way ANOVA) revealed that solid lipid and surfactant factors had a significant influence on particle size, PDI, and zeta potential (***p < 0.0001). According to the results, F1 and F6 formulations had desirable surface characterizations, physicochemical stability, and high E.E. %. The atomistic M.D. simulations confirmed that the F1 system (best) was more stable than the F31 system (worst).

Conclusion: The solid lipids: tripalmitin and compritol, stabilized with 4% tween 80 and 1% span 80, have produced stable NLC with the best surface characteristics that could be a promising formulation for the delivery of Q10. Atomistic M.D. simulation has confirmed the stability of F1 in comparison to F31.

Keywords: Coenzyme Q10; Q10-NLC; atomistic molecular dynamic.; entrapment efficiency (E.E.) %; nanostructured lipid carriers (NLC); stability study.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Lipids
Molecular Dynamics Simulation*
Spectroscopy, Fourier Transform Infrared
Surface-Active Agents*

Substances

coenzyme Q10
Surface-Active Agents
Lipids