Large-scale genomic survey and characterization of mcr genes carried by foodborne Cronobacter isolates

mSystems. 2023 Oct 26;8(5):e0045023. doi: 10.1128/msystems.00450-23. Epub 2023 Sep 11.

Abstract

Cronobacter is an emerging foodborne opportunistic pathogen, which can cause neonatal meningitis, bacteremia, and NEC by contaminating food. However, the entire picture of foodborne Cronobacter carriage of the mcr genes is not known. Here, we investigated the mcr genes of Cronobacter isolates by whole-genome sequencing and found 133 previously undescribed Cronobacter isolates carrying mcr genes. Further genomic analysis revealed that these mcr genes mainly belonged to the mcr-9 and mcr-10. Genomic analysis of the flanking structures of mcr genes revealed that two core flanking structures were prevalent in foodborne Cronobacter isolates, and the flanking structure carrying IS1R was found for the first time in this study.

Keywords: Cronobacter; flanking structures; foodborne; mcr genes; whole-genome sequencing.

MeSH terms

  • Cronobacter* / genetics
  • Genome
  • Genomics
  • Humans
  • Infant, Newborn
  • Phylogeny
  • Whole Genome Sequencing