Molecular cloning of the gene encoding the valyl-tRNA synthetase from Bacillus stearothermophilus

Gene. 1986;44(1):139-42. doi: 10.1016/0378-1119(86)90053-3.

Abstract

The valS gene from the thermophile Bacillus stearothermophilus encoding the valyl-tRNA synthetase has been cloned on a 13.8-kb plasmid. The gene product and its kinetic properties are comparable with those of the native enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acyl-tRNA Synthetases / genetics*
  • Cloning, Molecular*
  • Enzyme Stability
  • Genes*
  • Genes, Bacterial*
  • Geobacillus stearothermophilus / enzymology
  • Geobacillus stearothermophilus / genetics*
  • Kinetics
  • Plasmids
  • Valine-tRNA Ligase / genetics*
  • Valine-tRNA Ligase / metabolism

Substances

  • Amino Acyl-tRNA Synthetases
  • Valine-tRNA Ligase