Abstract
The valS gene from the thermophile Bacillus stearothermophilus encoding the valyl-tRNA synthetase has been cloned on a 13.8-kb plasmid. The gene product and its kinetic properties are comparable with those of the native enzyme.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acyl-tRNA Synthetases / genetics*
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Cloning, Molecular*
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Enzyme Stability
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Genes*
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Genes, Bacterial*
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Geobacillus stearothermophilus / enzymology
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Geobacillus stearothermophilus / genetics*
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Kinetics
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Plasmids
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Valine-tRNA Ligase / genetics*
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Valine-tRNA Ligase / metabolism
Substances
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Amino Acyl-tRNA Synthetases
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Valine-tRNA Ligase