Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR322

C J Burrell; P Mackay; P J Greenaway; P H Hofschneider; K Murray

doi:10.1038/279043a0

Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR322

Nature. 1979 May 3;279(5708):43-7. doi: 10.1038/279043a0.

Authors

C J Burrell, P Mackay, P J Greenaway, P H Hofschneider, K Murray

PMID: 377093
DOI: 10.1038/279043a0

Abstract

Fragments of hepatitis B virus DNA isolated from Dane particles have been inserted into the Escherichia coli plasmid pBR322 and cloned. Cells carrying the hybrid plasmid synthesise antigenic material that reacts specifically with antisera to hepatitis B viral antigens.

MeSH terms

Chromosome Mapping
DNA Restriction Enzymes / metabolism
DNA, Circular / genetics
DNA, Recombinant*
DNA, Viral / genetics*
Escherichia coli / genetics*
Genes, Viral
Hepatitis B virus / genetics*
Plasmids*

Substances

DNA, Circular
DNA, Recombinant
DNA, Viral
DNA Restriction Enzymes