Genome assembly and annotation of the Sharp-nosed Pit Viper Deinagkistrodon acutus based on next-generation sequencing data

GigaByte. 2023 Sep 4:2023:gigabyte88. doi: 10.46471/gigabyte.88. eCollection 2023.

Abstract

The study of the currently known >3,000 species of snakes can provide valuable insights into the evolution of their genomes. Deinagkistrodon acutus, also known as Sharp-nosed Pit Viper, one hundred-pacer viper or five-pacer viper, is a venomous snake with significant economic, medicinal and scientific importance. Widely distributed in southeastern China and South-East Asia, D. acutus has been primarily studied for its venom. Here, we employed next-generation sequencing to assemble and annotate a highly continuous genome of D. acutus. The genome size is 1.46 Gb; its scaffold N50 length is 6.21 Mb, the repeat content is 42.81%, and 24,402 functional genes were annotated. This study helps to further understand and utilize D. acutus and its venom at the genetic level.

对目前已知 3,000 余种蛇类的研究可为它们的基因组进化提供有价值的见 解。尖吻蝮,也被称为尖鼻蝮、百步蛇或五步蛇,是一种具有重要经济、医学 和科学价值的毒蛇。其广泛分布于中国东南部和东南亚,主要用于蛇毒研究。 本文采用二代测序技术,组装和注释了一个高度连续的尖吻蝮基因组。基因组 大小为 1.46 Gb; 其 scaffold N50 长度为 6.21 Mb,重复序列含量为 42.81%,共 注释出 24,402 个功能基因。本研究有助于在遗传水平上进一步认识和利用尖吻 蝮及其毒液。

Grants and funding

Our project was financially supported by the Guangdong Provincial Key Laboratory of Genome Read and Write (grant no. 2017B030301011). This work was also supported by China National GeneBank (CNGB).