The differentiation of N-butyl pentylone isomers using GC-EI-MS and NMR

Ruby E Liliedahl; Elise Hutzell; Madison Haley; Daniel P Predecki; J Tyler Davidson

doi:10.1016/j.forsciint.2023.111815

The differentiation of N-butyl pentylone isomers using GC-EI-MS and NMR

Forensic Sci Int. 2023 Oct:351:111815. doi: 10.1016/j.forsciint.2023.111815. Epub 2023 Sep 4.

Authors

Ruby E Liliedahl¹, Elise Hutzell², Madison Haley², Daniel P Predecki³, J Tyler Davidson⁴

Affiliations

¹ Department of Forensic Science, Sam Houston State University, Huntsville, TX, USA.
² Department of Chemistry, Shippensburg University, Shippensburg, PA, USA.
³ Department of Chemistry, Shippensburg University, Shippensburg, PA, USA. Electronic address: dppred@ship.edu.
⁴ Department of Forensic Science, Sam Houston State University, Huntsville, TX, USA. Electronic address: jtdavidson@shsu.edu.

PMID: 37713773
DOI: 10.1016/j.forsciint.2023.111815

Abstract

Forensic laboratories are faced with an ever-expanding seized drug landscape including the increasing prevalence of novel psychoactive substances (NPS), such as synthetic cathinones, that have varying potencies and scheduling. This study demonstrates a combined gas chromatography-electron ionization-mass spectrometry (GC-EI-MS) and nuclear magnetic resonance (NMR) spectroscopy approach for the differentiation of N-butyl pentylone isomers based on distinct retention times, characteristic EI mass spectra, and NMR characterization. Retention time reproducibility was assessed from 60 replicate measurements for each isomer over the course of a month. In addition, the effect of the mass spectrometer tune and the stability of an identified characteristic ion ratio using spectral data from ± 1 scan on either side of the peak apex were also statistically assessed using Welch's ANOVA testing. The presence of diastereomers for N-sec-butyl pentylone was identified using the developed GC-EI-MS method, which was confirmed using one-dimensional and two-dimensional NMR spectroscopy. The retention time reproducibility of the chromatographic method was ± 0.076% or less over the course of a month. An identified characteristic ion ratio between the abundance of the fragment ion at m/z 128 and the fragment ion at m/z 72 enabled the differentiation of the four N-butyl pentylone isomers, even when accounting for the effect of the mass spectrometer tune and mass spectral scans used to calculate the characteristic ion ratio. The 95% confidence interval mean abundance ratio of the fragment ions at m/z 128 and m/z 72 was 17.14 ± 0.14 for N-butyl pentylone, 6.44 ± 0.05 for N-isobutyl pentylone, 3.38 ± 0.02 for N-sec-butyl pentylone, and 0.75 ± 0.01 for N-tert-butyl pentylone. These results highlight the capabilities of a combined GC-EI-MS and NMR approach for the differentiation and characterization of synthetic cathinone isomers.

Keywords: Diastereomers; Forensic science; GC-EI-MS; NMR; Seized drugs; Synthetic cathinone isomers.