Recombinase-independent AAV for anterograde transsynaptic tracing

Mol Brain. 2023 Sep 15;16(1):66. doi: 10.1186/s13041-023-01053-7.

Abstract

Viral transsynaptic labeling has become indispensable for investigating the functional connectivity of neural circuits in the mammalian brain. Adeno-associated virus serotype 1 (AAV1) allows for anterograde transneuronal labeling and manipulation of postsynaptic neurons. However, it is limited to delivering an AAV1 expressing a recombinase which relies on using transgenic animals or genetic access to postsynaptic neurons. We reasoned that a strong expression level could overcome this limitation. To this end, we used a self-complementary AAV of serotype 1 (scAAV1) under a strong promoter (CAG). We demonstrated the anterograde transneuronal efficiency of scAAV1 by delivering a fluorescent marker in mouse retina-superior colliculus and thalamic-amygdala pathways in a recombinase-independent manner in the mouse brain. In addition to investigating neuronal connectivity, anterograde transsynaptic AAVs with a strong promoter may be suitable for functional mapping and imaging.

Keywords: AAV; Neural-circuit; Neuroanatomy; Transsynaptic; Viral-tracing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amygdala*
  • Animals
  • Animals, Genetically Modified
  • Brain*
  • Mammals
  • Mice
  • Recombinases

Substances

  • Recombinases

Supplementary concepts

  • Adeno-associated virus-1