Chlorogenic acid enhances PPARγ-mediated lipogenesis through preventing Lipin 1 nuclear translocation in Staphylococcus aureus-exposed bovine mammary epithelial cells

Ruiyuan Yao; Manshulin Wang; Yue Zhao; Qiang Ji; Xue Feng; Linfeng Bai; Lili Bao; Yanfeng Wang; Huifang Hao; Xihe Li; Zhigang Wang

doi:10.1016/j.bbalip.2023.159396

Chlorogenic acid enhances PPARγ-mediated lipogenesis through preventing Lipin 1 nuclear translocation in Staphylococcus aureus-exposed bovine mammary epithelial cells

Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Nov;1868(11):159396. doi: 10.1016/j.bbalip.2023.159396. Epub 2023 Sep 17.

Authors

Ruiyuan Yao¹, Manshulin Wang², Yue Zhao², Qiang Ji², Xue Feng³, Linfeng Bai², Lili Bao¹, Yanfeng Wang², Huifang Hao⁴, Xihe Li⁵, Zhigang Wang⁶

Affiliations

¹ State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China; School of Basic Medical Science, Inner Mongolia Medical University, Hohhot 010110, China.
² State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.
³ State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China; Hohhot No. 1 High School, Hohhot 010030, China.
⁴ State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China. Electronic address: 111981355@imu.edu.cn.
⁵ State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China; Research Center for Animal Genetic Resources of Mongolia Plateau, Inner Mongolia University, Hohhot 010070, China; Inner Mongolia SaiKexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot 011517, China. Electronic address: lixihe@saikexing.com.
⁶ State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China. Electronic address: lswzg@imu.edu.cn.

PMID: 37717905
DOI: 10.1016/j.bbalip.2023.159396

Abstract

Chlorogenic acid (CGA) as one of the most ubiquitously dietary polyphenolic compounds, has been reported to have various antimicrobial effects and exhibit strong anti-inflammatory ability. Staphylococcus aureus is a gram-positive bacterium that can induce mastitis. However, the mechanism through which S. aureus infection affects lipid synthesis and whether CGA have protective effect on S. aureus reduced lipid synthesis is not fully understood. In this study, the internalization of S. aureus reduced intracellular lipid droplet formation, decreased the levels of intracellular triacylglycerol, total cholesterol and 7 types of fatty acid and downregulated the expression of lipogenic genes FAS, ACC, and DGAT1 in bovine mammary epithelial cells (BMECs). In addition, we found that S. aureus intracellular infection attenuated mTORC1 activation resulting in Lipin 1 nuclear localization. Remarkablely, S. aureus infection-mediated repression of lipid synthesis related to the mTORC1 signaling and Lipin 1 nuclear localization can be alleviated by CGA. Thus, our findings provide a novel mechanism by which lipid synthesis is regulated under S. aureus infection and the protective effects of CGA on lipid synthesis in BMECs.

Keywords: Chlorogenic acid; Lipin 1; Lipogenesis; S. aureus; mTORC1.