A safety study on ultra‑high or moderate static magnetic fields combined with platycodin D against lung cancer

Oncol Lett. 2023 Sep 4;26(4):453. doi: 10.3892/ol.2023.14040. eCollection 2023 Oct.


Due to the serious side effects of chemotherapy drugs against lung cancer, and the antitumor properties and high safety of magnetic fields, the present study combined moderate or ultra-high intensity statics magnetic fields (SMFs) with platycodin D (PD) to explore the antitumor efficiency and biosafety. The antitumor effects of PD with or without moderate and ultra-high SMFs on A549 cells bearing mice were compared. Mouse body weight, food/water intake, hematology routine, blood biochemistry, tumor weight and tissues hematoxylin and eosin (H&E) staining were examined. Behavior was measured using the elevated plus maze, open field and vital signs tests. The combined targets of PD and SMFs were detected using RNA-sequencing (RNA-seq). The results showed that the antitumor effect of 22 Tesla (T) SMF group was 3.6-fold higher compared with that of the 2 mg/kg PD group (tumor growth inhibition=10.08%), while the antitumor effect of 150 mT SMF was only 1.56-fold higher compared with that of PD. Although PD reduced the food intake, there was no significant difference in body weight, water intake or food consumption among PD and SMF groups. Behavioral results indicated that PD ameliorated dysphoria in mice, but SMFs reduced this effect. However, no significant abnormalities were found in routine blood, blood biochemistry test, H&E staining or organ index, except renal index which was reduced by PD with or without SMFs. RNA-sequencing (RNA-seq) demonstrated that SMFs and PD synergistically targeted the expression of genes associated with tumor growth, inflammation and neurological disease. The present study showed the antitumor efficacy and biosafety of moderate or ultra-high SMF combined with PD, which exhibited only few side effects in the treatment of lung cancer, thus supporting further research for the clinical application of magnetic fields.

Keywords: biosafety; lung cancer; magnetic resonance imaging; platycodin D; static magnetic field.

Grants and funding

This work was funded by Anhui Provincial Natural Science Research Project of Higher Education (grant nos. KJ2021A0925 and KJ2020A0095), Anhui Provincial Natural Science Foundation (grant no. 2008085MC105), Research Foundation for Talented Scholars of Hefei Normal University (grant nos. 2020rcjj46 and 2022rcjj21) and Hefei Institutes of Physical Science Director's Fund (grant no. YZJJ2023QN43).