Integrated 16S rRNA sequencing and nontargeted metabolomics analysis to reveal the mechanisms of Yu-Ye Tang on type 2 diabetes mellitus rats

Ziang Ma; Wenjuan Sun; Lixin Wang; Yuansong Wang; Baochao Pan; Xiuhai Su; Hanzhou Li; Hui Zhang; Shuquan Lv; Hongwu Wang

doi:10.3389/fendo.2023.1159707

Integrated 16S rRNA sequencing and nontargeted metabolomics analysis to reveal the mechanisms of Yu-Ye Tang on type 2 diabetes mellitus rats

Front Endocrinol (Lausanne). 2023 Sep 5:14:1159707. doi: 10.3389/fendo.2023.1159707. eCollection 2023.

Authors

Ziang Ma¹, Wenjuan Sun², Lixin Wang², Yuansong Wang², Baochao Pan¹, Xiuhai Su², Hanzhou Li³, Hui Zhang¹, Shuquan Lv², Hongwu Wang⁴

Affiliations

¹ Graduate School of Hebei University of Chinese Medicine, Shijiazhuang, China.
² Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine of Hebei Province Affiliated to Hebei University of Chinese Medicine, Cangzhou, China.
³ College of Integrated Chinese and Western Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
⁴ College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Abstract

Introduction: Yu-Ye Tang (YYT) is a classical formula widely used in treatment of type 2 diabetes mellitus (T2DM). However, the specific mechanism of YYT in treating T2DM is not clear.

Methods: The aim of this study was to investigate the therapeutic effect of YYT on T2DM by establishing a rat model of T2DM. The mechanism of action of YYT was also explored through investigating gut microbiota and serum metabolites.

Results: The results indicated YYT had significant therapeutic effects on T2DM. Moreover, YYT could increase the abundance of Lactobacillus, Candidatus_Saccharimonas, UCG-005, Bacteroides and Blautia while decrease the abundance of and Allobaculum and Desulfovibrio in gut microbiota of T2DM rats. Nontargeted metabolomics analysis showed YYT treatment could regulate arachidonic acid metabolism, alanine, aspartate and glutamate metabolism, arginine and proline metabolism, glycerophospholipid metabolism, pentose and glucuronate interconversions, phenylalanine metabolism, steroid hormone biosynthesis, terpenoid backbone biosynthesis, tryptophan metabolism, and tyrosine metabolism in T2DM rats.

Discussion: In conclusion, our research showed that YYT has a wide range of therapeutic effects on T2DM rats, including antioxidative and anti-inflammatory effects. Furthermore, YYT corrected the altered gut microbiota and serum metabolites in T2DM rats. This study suggests that YYT may have a therapeutic impact on T2DM by regulating gut microbiota and modulating tryptophan and glycerophospholipid metabolism, which are potential key pathways in treating T2DM.

Keywords: Yu-Ye Tang; glycerophospholipid metabolism; gut microbiota; tryptophan metabolism; type 2 diabetes mellitus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diabetes Mellitus, Type 2* / drug therapy
Diabetes Mellitus, Type 2* / genetics
Glycerophospholipids
Metabolomics
RNA, Ribosomal, 16S
Rats
Tryptophan

Substances

RNA, Ribosomal, 16S
Tryptophan
Glycerophospholipids

Grants and funding

This research was funded by Construction Project of Workshop of Prestigious Chinese Physician in Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine, grant number 2022-75; Tianjin Municipal Education Commission (Natural Key) Scientific Research Programme Projects, grant number 2021ZD010.