Multi-scale cellular imaging of DNA double strand break repair

Tim Heemskerk; Gerarda van de Kamp; Jeroen Essers; Roland Kanaar; Maarten W Paul

doi:10.1016/j.dnarep.2023.103570

Multi-scale cellular imaging of DNA double strand break repair

DNA Repair (Amst). 2023 Nov:131:103570. doi: 10.1016/j.dnarep.2023.103570. Epub 2023 Sep 19.

Authors

Tim Heemskerk¹, Gerarda van de Kamp¹, Jeroen Essers², Roland Kanaar¹, Maarten W Paul³

Affiliations

¹ Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, the Netherlands.
² Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, the Netherlands; Department of Vascular Surgery, Erasmus University Medical Center, Rotterdam, the Netherlands; Department of Radiotherapy, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, the Netherlands.
³ Department of Molecular Genetics, Oncode Institute, Erasmus MC Cancer Institute, Erasmus University Medical Center, Rotterdam, the Netherlands. Electronic address: m.w.paul@erasmusmc.nl.

PMID: 37734176
DOI: 10.1016/j.dnarep.2023.103570

Abstract

Live-cell and high-resolution fluorescence microscopy are powerful tools to study the organization and dynamics of DNA double-strand break repair foci and specific repair proteins in single cells. This requires specific induction of DNA double-strand breaks and fluorescent markers to follow the DNA lesions in living cells. In this review, where we focused on mammalian cell studies, we discuss different methods to induce DNA double-strand breaks, how to visualize and quantify repair foci in living cells., We describe different (live-cell) imaging modalities that can reveal details of the DNA double-strand break repair process across multiple time and spatial scales. In addition, recent developments are discussed in super-resolution imaging and single-molecule tracking, and how these technologies can be applied to elucidate details on structural compositions or dynamics of DNA double-strand break repair.

Keywords: DNA repair kinetics; Live cell microscopy; Multiscale imaging; Super-resolution microscopy.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA
DNA Breaks, Double-Stranded*
DNA Repair*
Mammals / genetics
Microscopy, Fluorescence / methods
Single Molecule Imaging

Substances

DNA