Analysis of the response mechanisms of Pinellia ternata to terahertz wave stresses using transcriptome and metabolic data

Dongdong Wang; Surendra Sarsaiya; Xu Qian; Leilei Jin; Fuxing Shu; Chuanyou Zhang; Jishuang Chen

doi:10.3389/fpls.2023.1227507

Analysis of the response mechanisms of Pinellia ternata to terahertz wave stresses using transcriptome and metabolic data

Front Plant Sci. 2023 Sep 12:14:1227507. doi: 10.3389/fpls.2023.1227507. eCollection 2023.

Authors

Dongdong Wang¹, Surendra Sarsaiya², Xu Qian¹, Leilei Jin¹, Fuxing Shu¹, Chuanyou Zhang³, Jishuang Chen^{1

2}

Affiliations

¹ College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu, China.
² Bioresource Institute for Healthy Utilization, Zunyi Medical University, Zunyi, Guizhou, China.
³ Nanjing Kuncan Technology Co., Nanjing, China.

Abstract

Pinellia ternata (Thunb.) Breit. (Araceae), a significant medicinal plant, has been used to treat various diseases for centuries. Terahertz radiation (THZ) is located between microwaves and infrared rays on the electromagnetic spectrum. THZ possesses low single-photon energy and a spectral fingerprint, but its effects on plant growth have not yet been investigated. The study's primary objective was to examine the transcriptome and metabolome databases of the SY line to provide a new perspective for identifying genes associated with resistance and growth promotion and comprehending the underlying molecular mechanism. Variations in the biological characteristics of P. ternata grown under control and experimental conditions were analyzed to determine the effect of THZ. Compared with the control group, phenotypic variables such as leaf length, petiole length, number of leaves, leaf petiole diameter, and proliferation coefficient exhibited significant differences. P. ternata response to THZ was analyzed regarding the effects of various coercions on root exudation. The experimental group contained considerably more sugar alcohol than the control group. The transcriptome analysis revealed 1,695 differentially expressed genes (DEGs), including 509 upregulated and 1,186 downregulated genes. In the KEGG-enriched plant hormone signaling pathway, there were 19 differentially expressed genes, 13 of which were downregulated and six of which were upregulated. In the metabolomic analysis, approximately 416 metabolites were uncovered. There were 112 DEMs that were downregulated, whereas 148 were upregulated. The P. ternata leaves displayed significant differences in phytohormone metabolites, specifically in brassinolide (BR) and abscisic acid (ABA). The rise in BR triggers alterations in internal plant hormones, resulting in faster growth and development of P. ternata. Our findings demonstrated a link between THZ and several metabolic pathway processes, which will enhance our understanding of P. ternata mechanisms.

Keywords: Pinellia ternata; metabonomic; plant hormones; terahertz; transcriptome.

Grants and funding

This work was supported by Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (Grant Number: XTD1825). National Natural Science Foundation of China (82373981).