A 3D printed plastic frame deeply impacts yeast cell growth

Esther Molina-Menor; Àngela Vidal-Verdú; Carlos Gomis-Olcina; Juli Peretó; Manuel Porcar

doi:10.3389/fbioe.2023.1250667

A 3D printed plastic frame deeply impacts yeast cell growth

Front Bioeng Biotechnol. 2023 Sep 12:11:1250667. doi: 10.3389/fbioe.2023.1250667. eCollection 2023.

Authors

Esther Molina-Menor¹, Àngela Vidal-Verdú¹, Carlos Gomis-Olcina¹, Juli Peretó^{1

2}, Manuel Porcar^{1

3}

Affiliations

¹ Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, Spain.
² Departament de Bioquímica i Biologia Molecular, Universitat de València, Burjassot, Spain.
³ Darwin Bioprospecting Excellence SL, Parc Científic Universitat de València, Valencia, Spain.

Abstract

Solid State Fermentation (SSF) processes have been explored for yeast growth and protein and metabolites production. However, most of these processes lack standardization. In this work, we present a polylactic acid (PLA) 3D printed matrix that dramatically enhances yeast growth when embedded in liquid media compared to equivalent static cultures, and changes yeast expression patterns at the proteome level (data are available via ProteomeXchange with identifier PXD043759). Moreover, differences in sugar assimilation and ethanol production, as the main product of alcoholic fermentation, are observed. Our results suggest that these matrixes may be useful for a vast range of biotechnological applications based on yeast fermentation.

Keywords: Saccharomyces cerevisiae; biomass production; proteomics; solid state fermentation; yeast.

Grants and funding

Financial support from the Spanish Government (Grant SETH, Reference: RTI2018-095584-B-C41-42-43-44 co-financed by FEDER funds and Ministerio de Ciencia, Innovación y Universidades), the European CSA on biological standardization BIOROBOOST (EU grant number 820699) and Micro4Biogas (European Commission H2020 Program Ref. Grant Agreement ID 101000470) is acknowledged. EM-M and ÀV-V are funded with a Formación de Profesorado Universitario (FPU) grant from the Spanish Government (Ministerio de Ciencia, Innovación y Universidades), with references FPU17/04184 and FPU18/02578, respectively.