KDM2B regulates inflammation and oxidative stress of sepsis via targeting NF-κB and AP-1 pathways

Xin Li; Xinyu Tian; Dongliang Zhang

doi:10.1002/iid3.985

KDM2B regulates inflammation and oxidative stress of sepsis via targeting NF-κB and AP-1 pathways

Immun Inflamm Dis. 2023 Sep;11(9):e985. doi: 10.1002/iid3.985.

Authors

Xin Li¹, Xinyu Tian¹, Dongliang Zhang¹

Affiliation

¹ Department of Nephrology, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China.

Abstract

Backgrounds: The kidney is an easily affected organ with sepsis which is a main underlying cause of acute kidney injury (AKI). Histone-modifying lysine-specific demethylase 2B (KDM2B) is involved in numerous pathological processes, such as cell senescence and tumor development. However, the role of KDM2B in sepsis-induced AKI is unclear.

Objects: To investigate the role of KDM2B on cell viability, inflammation and oxidative stress of sepsis-associated AKI, and the involved signaling pathways.

Methods: An AKI model in vitro was established through lipopolysaccharide (LPS)-induction in HK-2 cells. Western blots were performed to evaluate the expression of KDM2B, cyclooxygenase 2 (COX2), inducible nitric oxide synthase (iNOS), p65, c-Jun and c-Fos, as well as p65 phosphorylation. Cell viability was measured using CCK-8 kit. ELISA was performed to analyze the production of layered double hydroxide (LDH), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-18, vascular cell adhesion molecule-1 (VCAM-1), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), and H₂ O₂ . The qPCR was used to evaluate the transcription level of TNF-α, IL-1β, IL-18, and VCAM-1.

Results: KDM2B knockdown alleviated LPS-induced cytotoxicity, decreased LDH release, and improved cell viability. KDM2B knockdown reduced concentration of inflammation-related molecules including TNF-α, IL-1β, IL-18, and VCAM-1, and inhibited their transcription. Moreover, KDM2B knockdown promoted the quantity of SOD and GSH, while declined the production of MDA, H₂ O₂ , COX2, and iNOS. Further, KDM2B played a role in LPS-induced HK-2 cell injury by activating nuclear factor κB (NF-κB) and activator protein 1 (AP-1) pathways.

Conclusion: KDM2B knockdown reduced cytotoxicity, inflammation and oxidative stress in LPS-induced AKI via inhibiting NF-κB and AP-1 pathways, indicating KDM2B may be a promising therapeutic target for the treatment of sepsis-associated AKI.

Keywords: AP-1; KDM2B; NF-κB; cell proliferation; inflammation; oxidative stress; sepsis.