pH-responsive multi-network composite cellulose-based hydrogels for stable delivery of oral IgY-Fab fragments

Food Chem. 2024 Mar 1:435:137567. doi: 10.1016/j.foodchem.2023.137567. Epub 2023 Sep 26.


Yolk immunoglobulin (IgY) is perfect supplement to mammalian immunoglobulin G in passive immune protection but with poor delivery stability. This work succeeded in pH-responsive oral delivery of IgY-Fab fragments with cellulose based multi-network composite hydrogels. Data displayed that the hydrogel 2 showed superior mechanical properties and load performance (encapsulation efficiency of 99.25% and loading capacity of 45.11 mg/100 mg). The stability of the released Fab was confirmed by HPLC with Fab purity up to 79.65% at the end of digestion. The FTIR spectra revealed the potential interactions between Fab and the hydrogel matrix of the formation of hydrogen bonds or electrostatic interactions between the groups of -OH, -CH2, and -COO-. The excellent rehydration of the hydrogels wouldn't be impacted by low-temperature freeze drying. In sum, this work is of great significance to the development of Fab-themed health-care food, intensive processing of poultry eggs and the economic construction of related industries.

Keywords: Fab fragment; Multi-network composite hydrogel; Stable oral delivery; pH response release.

MeSH terms

  • Animals
  • Cellulose* / chemistry
  • Hydrogels / chemistry
  • Hydrogen-Ion Concentration
  • Immunoglobulin Fab Fragments*
  • Mammals


  • IgY
  • Immunoglobulin Fab Fragments
  • Cellulose
  • Hydrogels