Novel filter-press single-step cleanup approach facilitated rapid screening and accurate quantification of 112 veterinary drugs in aquatic products

Jincheng Li; Ruidong Zhou; Guangxin Yang; Essy Kouadio Fodjo; Tengwang Feng; Huiwu Sun; Dongmei Huang; Cong Kong; Huan Liu

doi:10.1016/j.fochx.2023.100846

Novel filter-press single-step cleanup approach facilitated rapid screening and accurate quantification of 112 veterinary drugs in aquatic products

Food Chem X. 2023 Aug 19:19:100846. doi: 10.1016/j.fochx.2023.100846. eCollection 2023 Oct 30.

Authors

Jincheng Li¹, Ruidong Zhou^{1

2}, Guangxin Yang³, Essy Kouadio Fodjo⁴, Tengwang Feng^{1

2}, Huiwu Sun¹, Dongmei Huang³, Cong Kong³, Huan Liu¹

Affiliations

¹ Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Beijing 100141, PR China.
² College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, PR China.
³ Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China.
⁴ Laboratory of Constitution and Reaction of Matter (Physical Chemistry), Université Felix Houphou-et-Boigny, 22 BP 582 Abidjan 22, Cote d'Ivoire.

Abstract

In this study, a novel filter-press cleanup column was developed as a single-step cleanup approach for the rapid screening and quantification of 112 veterinary drugs in fish samples. Fish muscle samples were extracted with acetonitrile and ethyl acetate, sequentially. After concentration and reconstitution, N-propylethylenediamine (PSA) sorbent, packed in filter-press column, allows rapid single-step cleanup operation, while UHPLC-Q-Orbitrap-HRMS provides high-precision mass information in multi-residue screening. Under optimum settings, the detection and quantification limits were validated at 0.5 and 2.0 μg·kg^-1, for all analytes, respectively. The ranges of recoveries were from 35.3 to 138.4%. Most of these target analytes (82%) could be measured with recoveries between 60 and 130%, and intra-day RSDs ranging from 1.9 to 26.1%. This method was further applied to evaluate the residual of veterinary drugs in fish samples from four cities in China, and results demonstrated its practicability for multi-residue monitoring veterinary residues for food safety administration.

Keywords: Batch purification; Drug residues; Fish; Sample pretreatment; UHPLC-Q-Orbitrap-HRMS.