Poly(ADP-ribosyl)ation of DNA polymerase beta in vitro

Biochem Biophys Res Commun. 1986 Oct 30;140(2):666-73. doi: 10.1016/0006-291x(86)90783-7.


DNA polymerase beta purified from bovine thymus is markedly inhibited when incubated in a reconstituted poly(ADP-ribosyl)ating reaction system. Analyses of the reaction product synthesized in this system by SDS-polyacrylamide gel electrophoresis and subsequent fluorography of the gel indicated that ADP-ribose is covalently attached to DNA polymerase beta molecule (Mr = 44,000).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Polymerase I / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Hydrogen-Ion Concentration
  • Nucleoside Diphosphate Sugars / metabolism*
  • Photofluorography
  • Poly Adenosine Diphosphate Ribose / metabolism*
  • Protein Binding


  • Nucleoside Diphosphate Sugars
  • Poly Adenosine Diphosphate Ribose
  • DNA Polymerase I