Characterization of neuromedin U like immunoreactivity in rat, porcine, guinea-pig and human tissue extracts using a specific radioimmunoassay

Biochem Biophys Res Commun. 1986 Nov 14;140(3):1127-34. doi: 10.1016/0006-291x(86)90752-7.


Two novel bioactive peptides termed neuromedin U-8 and neuromedin U-25 have recently been isolated from porcine spinal cord but nothing is known of their occurrence and molecular forms in other species. Following gel permeation chromatography, a specific radioimmunoassay detected only a single molecular form of neuromedin U-like immunoreactivity (NmU-LI) in rat, porcine and human central nervous system and gastrointestinal tract. Only guinea pig tissue extracts revealed two molecular forms of NmU-Li. Reverse phase high performance liquid chromatographic (HPLC) analysis demonstrated that porcine NmU-LI co-eluted with synthetic neuromedin U-25 standard. Human and rat NmU-LI however, was more hydrophobic on HPLC thus indicating species differences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Central Nervous System / analysis
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Digestive System / analysis
  • Guinea Pigs
  • Humans
  • Male
  • Neuropeptides / analysis*
  • Radioimmunoassay
  • Rats
  • Species Specificity
  • Swine


  • Neuropeptides
  • neuromedin U 8
  • neuromedin U 25