OMIP-099: 31-color spectral flow cytometry panel to investigate the steady-state phenotype of human T cells

Cytometry A. 2024 Jan;105(1):10-15. doi: 10.1002/cyto.a.24799. Epub 2023 Oct 9.


We have developed a 31-color panel to define the steady-state phenotype of T cells in human peripheral blood (Table 1). The panel presented here was optimized using cryopreserved peripheral blood mononuclear cells (PBMC). The markers included in this panel were chosen in order to characterize the steady-state phenotype of T cells and includes markers (CD45RA, CD45RO, CCR7, CD95) to distinguish the main subsets (e.g., naïve, TEM , TCM , TEMRA , TSCM etc.) of CD4, CD8, and γδ T cells. This panel also includes markers for the identification of differentiation status (CD27, CD28), activation/antigen experience status (CD11a, CD49d, CD38, HLA-DR, CD56, and CD39), co-inhibitory marker expression (PD-1, TIM-3), and CD4 T helper subsets (CXCR3, CXCR5, CCR4, CCR6, Foxp3, CD25, and CD127). This optimized panel provides a broad assessment of the steady-state phenotype of human T cells.

Keywords: CD4 T cells; CD8 T cells; T cells; cytek aurora; gamma-delta T cells; spectral flow cytometry.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Flow Cytometry
  • Humans
  • Leukocyte Common Antigens / metabolism
  • Leukocytes, Mononuclear* / metabolism
  • Phenotype
  • T-Lymphocyte Subsets
  • T-Lymphocytes* / metabolism


  • Leukocyte Common Antigens