Type I Lipoteichoic Acid (LTA) Detection by Western Blot

Methods Mol Biol. 2024:2727:95-106. doi: 10.1007/978-1-0716-3491-2_8.


Type I lipoteichoic acid (LTA) is a glycerol phosphate polymer found in the cell envelope of diverse Gram-positive bacteria including Staphylococcus aureus, Bacillus subtilis, and Listeria monocytogenes. The polymer is linked by a lipid anchor to the outer leaflet of the bacterial membrane and in some bacteria can also be shed and detected in the culture supernatant. Here, we describe a simple and rapid western blot method for the detection of Type I LTA in bacterial cell extracts and culture supernatant fractions using a polyglycerol phosphate specific monoclonal LTA antibody.

Keywords: Bacterial cell extracts; Culture supernatant samples; Monoclonal polyglycerol phosphate specific LTA antibody; SDS-PAGE; Type I LTA; Western blot.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Lipopolysaccharides* / metabolism
  • Phosphates
  • Polymers
  • Teichoic Acids* / metabolism


  • lipoteichoic acid
  • Lipopolysaccharides
  • Teichoic Acids
  • Polymers
  • Phosphates