Novel plant disease detection techniques-a brief review

Mol Biol Rep. 2023 Nov;50(11):9677-9690. doi: 10.1007/s11033-023-08838-y. Epub 2023 Oct 12.

Abstract

Plant pathogens cause severe losses to agricultural yield worldwide. Tracking plant health and early disease detection is important to reduce the disease spread and thus economic loss. Though visual scouting has been practiced from former times, detection of asymptomatic disease conditions is difficult. So, DNA-based and serological methods gained importance in plant disease detection. The progress in advanced technologies challenges the development of rapid, non-invasive, and on-field detection techniques such as spectroscopy. This review highlights various direct and indirect ways of detecting plant diseases like Enzyme-linked immunosorbent assay, Lateral flow assays, Polymerase chain reaction, spectroscopic techniques and biosensors. Although these techniques are sensitive and pathogen-specific, they are more laborious and time-intensive. As a consequence, a lot of interest is gained in in-field adaptable point-of-care devices with artificial intelligence-assisted pathogen detection at an early stage. More recently computer-aided techniques like neural networks are gaining significance in plant disease detection by image processing. In addition, a concise report on the latest progress achieved in plant disease detection techniques is provided.

Keywords: DNA; Image processing; Neural networks; Plant disease; Volatile organic compounds.

Publication types

  • Review

MeSH terms

  • Artificial Intelligence*
  • Plant Diseases
  • Plants* / genetics
  • Polymerase Chain Reaction / methods