A research on the influence of G-CSF mobilization on donor's peripheral blood MDSCs and its relationship with patient prognosis

Abstract

Objective: To discuss the effects of mobilization of healthy donors with granulocyte colony-stimulating factor (G-CSF) on the absolute values and functions of myeloid-derived suppressor cells (MDSCs) and subpopulations of M-MDSCs and P-MDSCs in their peripheral blood. In addition, this study also aims to investigate the impacts of the adoptively transferred MDSCs from the grafts to the patients on their prognosis and immune reconstitution.

Methods: The selection of 72 donors and 72 patients were conducted for allogeneic hematopoietic stem cell transplantation (allo-HSCT) from August 2022 to December 2022 at Lu Daopei Hospital in Beijing, China. Statistical calculations were performed by Wilcoxon signed-rank test, Kruskal Wallis test, χ² test, Kaplan Meier test, and log-rank test to analyze the data.

Results & conclusion: G-CSF induced significant amplification of MDSCs in the peripheral blood of donors in percentage and absolute values. Whether the level of P-MDSCs in patients conducted for the adoptive transfer of P - MDSCs is higher than 3.7× 10⁷/kg or lower than 1.4× 10⁷/kg leads to a poor prognosis of the patients. Ensuring a balanced state of MDSCs is crucial for effective immunotherapy. Transferring a high level of MDSCs from the graft to the patient's body is advantageous for the development of MDSCs while simultaneously inhibiting the proliferation of lymphocyte subgroups.

Keywords: Allogeneic hematopoietic stem cell transplantation (allo-HSCT); Flow cytometry; Granulocyte colony-stimulating factor (G-CSF); Myeloid-derived suppressor cells (MDSCs); Prognosis.