Mutants in transmission of chemotactic signals from two independent receptors of E. coli

Cell. 1979 Mar;16(3):617-25. doi: 10.1016/0092-8674(79)90035-7.

Abstract

We have characterized chemotactic mutants of E. coli that appear to be defective in a common linkage of two independent receptors to the central chemotactic components. The mutants do not respond to gradients of ribose or galactose and thus are called trg (taxis to ribose and galactose), after Ordal and Adler (1974b). These trg mutants are indistinguishable from their parent in tactic response to other attractants, swimming pattern, growth rates, and transport of ribose and galactose. The mutant cells contain the usual amounts of ribose and galactose receptors, and those proteins function normally in their other role, transport of their respective ligands. The mutations, generated by insertion of translocatable drug-resistance elements (transposons)8 are located near 31 min on the map of the E. coli chromosome, a locus far removed from the genes coding for the ribose and galactose receptors. Trg mutants do not resemble either specific receptor mutants or che mutants. The nature of the requirement for the trg product in the response to ribose and galactose is not defined, but evidence for interference of tactic signals from the ribose and galactose receptors (Strange and Koshland, 1976) supports the idea that the product functions directly in the transmission of tactic signals from the two receptors to the flagella.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biological Transport
  • Carrier Proteins / physiology
  • Chemotaxis*
  • Escherichia coli / genetics
  • Escherichia coli / physiology*
  • Galactose / metabolism
  • Locomotion
  • Mutation
  • Receptors, Drug / physiology
  • Ribose / metabolism
  • Translocation, Genetic

Substances

  • Carrier Proteins
  • Receptors, Drug
  • Ribose
  • Galactose