SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures

Maria Victoria Humbert; Christopher J McCormick; Cosma Mirella Spalluto

doi:10.1007/978-1-0716-3507-0_2

SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures

Methods Mol Biol. 2024:2725:27-53. doi: 10.1007/978-1-0716-3507-0_2.

Authors

Maria Victoria Humbert¹, Christopher J McCormick^{2

3}, Cosma Mirella Spalluto^{4

5}

Affiliations

¹ Medical Research Council Toxicology Unit, School of Biological Sciences, University of Cambridge, Cambridge, UK.
² Faculty of Medicine, School of Clinical and Experimental Sciences, University of Southampton, Southampton, UK.
³ Southampton NIHR Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust, Southampton, UK.
⁴ Faculty of Medicine, School of Clinical and Experimental Sciences, University of Southampton, Southampton, UK. C.Spalluto@soton.ac.uk.
⁵ Southampton NIHR Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust, Southampton, UK. C.Spalluto@soton.ac.uk.

PMID: 37856016
DOI: 10.1007/978-1-0716-3507-0_2

Abstract

Respiratory epithelial cells fail to exhibit natural phenotypic and morphological characteristics when grown in standard cell culture conditions. To better understanding respiratory pathogen host-cell interactions in the airways, one approach is to instead grow and differentiate these cells at an air-liquid interface (ALI). This chapter provides the working protocols used in our lab for producing ALI cultures, infecting them with SARS-CoV-2 and monitoring viral replication.

Keywords: ACE-2; Air-liquid interface culture; Cilia; Primary nasal epithelial cells; SARS-CoV-2.

MeSH terms

COVID-19*
Cell Culture Techniques / methods
Epithelial Cells
Humans
Respiratory System
SARS-CoV-2