Zinc oxide-ozonated olive oil as a new root canal filling material in primary molars: a clinical randomized controlled trial

Clin Oral Investig. 2023 Dec;27(12):7395-7405. doi: 10.1007/s00784-023-05329-z. Epub 2023 Oct 21.


Objectives: The complex root canal anatomy of primary teeth keeps it very tricky to attain appropriate cleansing by biomechanical instrumentation, so obtaining an obturating material with excellent antimicrobial properties is a challenge in current clinical pulpectomy practice. So, this study aimed to assess the clinical and radiographic performance of zinc oxide-ozonated olive oil as a primary root canal filling material.

Materials and methods: Ninety non-vital primary molars in children ranging from 4 to 8 years were allocated into three groups in which root canals were filled with zinc oxide-ozonated olive oil, zinc oxide-olive oil, and zinc oxide-eugenol (ZOE) according to each group after pulpectomy procedure. Clinical and radiographic evaluations were done at 3-, 6-, and 12-month follow-up periods. Statistical analysis was performed for the collected data.

Results: All study groups showed a significant improvement regarding clinical signs and symptoms during follow-up periods. Ozonated-olive oil group revealed a significant increase in furcation radiodensity and a decrease in periodontal ligament space at 3-, 6-, and 12-month follow-up intervals compared to other groups.

Conclusion: Zinc oxide-ozonated olive oil and zinc oxide-olive oil paste had shown good clinical and radiographic success for primary teeth pulpectomy.

Clinical relevance: The intricate torturous primary root canal anatomy, in addition to the child's negative behavior, interferes with the complete debridement, so the long-lasting antibacterial effect of the primary root canal filling material aids in the pulpectomy success.

Keywords: Ozonated olive oil; Primary molars; Pulpectomy; Zinc-oxide eugenol.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Child
  • Child, Preschool
  • Humans
  • Molar / surgery
  • Olive Oil
  • Pulpectomy / methods
  • Root Canal Filling Materials* / pharmacology
  • Tooth, Deciduous
  • Zinc Oxide* / pharmacology
  • Zinc Oxide-Eugenol Cement / pharmacology


  • Olive Oil
  • Root Canal Filling Materials
  • Zinc Oxide
  • Zinc Oxide-Eugenol Cement