An isothermal CRISPR-based diagnostic assay for Neisseria gonorrhoeae and Chlamydia trachomatis detection

Hao Luo; Lihong Zeng; Xiaona Yin; Yuying Pan; Jianjiang Yang; Mingjing Liu; Xiaolin Qin; Zhanqin Feng; Wentao Chen; Heping Zheng

doi:10.1128/spectrum.00464-23

An isothermal CRISPR-based diagnostic assay for Neisseria gonorrhoeae and Chlamydia trachomatis detection

Microbiol Spectr. 2023 Dec 12;11(6):e0046423. doi: 10.1128/spectrum.00464-23. Epub 2023 Oct 26.

Authors

Hao Luo^#^{1

2}, Lihong Zeng^#^{1

2}, Xiaona Yin^#^{1

2}, Yuying Pan^{1

2}, Jianjiang Yang^{1

2}, Mingjing Liu³, Xiaolin Qin^{1

2}, Zhanqin Feng^{1

2}, Wentao Chen^{1

2}, Heping Zheng^{1

2}

Affiliations

¹ Dermatology Hosptial, Southern Medical University , Guangzhou, China.
² Guangzhou Key Laboratory for Sexually Transmitted Diseases Control , Guangzhou, China.
³ Medical College, China Three Gorges University , Yichang, China.

^# Contributed equally.

Abstract

A method for Neisseria gonorrhoeae (NG)/Chlamydia trachomatis (CT) detection is developed using multiplex-recombinase polymerase amplification and Cas12a/Cas13a. This method can detect NG and CT simultaneously with high sensitivity and specificity. This method has great potential to be further developed into larger-scale screening and point-of-care testing (POCT).

Keywords: CRISPR‒Cas12a; CRISPR‒Cas13a; Chlamydia trachomatis; Neisseria gonorrhoeae; coinfection; dual-target detection.

MeSH terms

Chlamydia Infections* / diagnosis
Chlamydia trachomatis / genetics
Gonorrhea* / diagnosis
Humans
Neisseria gonorrhoeae / genetics
Sensitivity and Specificity

Abstract

MeSH terms

Grants and funding