Development of Monoclonal Antibodies Specific to Either CD300AR111 or CD300AQ111 or Both

Hitoshi Koizumi; Chigusa Nakahashi-Oda; Wenxin Lyu; Yumi Yamashita-Kanemaru; Keiji Tabuchi; Kazuko Shibuya; Akira Shibuya

doi:10.1089/mab.2023.0017

Development of Monoclonal Antibodies Specific to Either CD300A^R111 or CD300A^Q111 or Both

Monoclon Antib Immunodiagn Immunother. 2023 Oct;42(5):182-185. doi: 10.1089/mab.2023.0017.

Authors

Hitoshi Koizumi^{1

2}, Chigusa Nakahashi-Oda^{1

3}, Wenxin Lyu¹, Yumi Yamashita-Kanemaru⁴, Keiji Tabuchi², Kazuko Shibuya^{1

3}, Akira Shibuya^{1

4

5}

Affiliations

¹ Department of Immunology, Institute of Medicine, University of Tsukuba, Tsukuba, Japan.
² Department of Otolaryngology-Head and Neck Surgery, Institute of Medicine, University of Tsukuba, Tsukuba, Japan.
³ R&D Center for Innovative Drug Discovery, University of Tsukuba, Ibaraki, Japan.
⁴ TNAX Biopharma Corporation, Tsukuba, Ibaraki, Japan.
⁵ Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), Tsukuba, Japan.

PMID: 37902989
DOI: 10.1089/mab.2023.0017

Abstract

CD300A is a member of the CD300 immunoglobulin (Ig)-like receptor family consisting of eight molecules in humans, all of which contain one Ig-like domain in the extracellular portion. Upon binding its ligand phosphatidylserine or phosphatidylethanolamine, CD300A mediates an inhibitory signal through the immunoreceptor tyrosine-based inhibitory motif in the cytoplasmic portion. The CD300 family molecules are highly homologous to each other. In addition, CD300A has a single nucleotide polymorphism (rs2272111), which is a nonsense mutation encoding glutamine (CD300A^Q111) instead of arginine (CD300A^R111) at residue 111 in the Ig-like domain of CD300A. In this study, we successfully generated monoclonal antibodies (mAbs) specific to either CD300A^R111 or CD300A^Q111 or both. These mAbs are useful for the analysis of CD300A genotype by flow cytometry and the development of an antibody drug for the treatment of various diseases.

Keywords: CD300A; SNP; human peripheral blood mononuclear cells; immune inhibitory receptor; monoclonal antibodies.

MeSH terms

Antibodies, Monoclonal*
Antigens, CD / genetics
Humans
Receptors, Immunologic* / chemistry
Receptors, Immunologic* / genetics

Substances

Antibodies, Monoclonal
Receptors, Immunologic
CD300A protein, human
Antigens, CD