Demineralized dentin matrix promotes gingival healing in alveolar ridge preservation of premolars extracted for orthodontic reason: a split-mouth study

Xiaofeng Xu; Dongsheng Peng; Bowei Zhou; Kaijin Lin; Siyi Wang; Wei Zhao; Minqian Zheng; Jin Yang; Jianbin Guo

doi:10.3389/fendo.2023.1281649

Demineralized dentin matrix promotes gingival healing in alveolar ridge preservation of premolars extracted for orthodontic reason: a split-mouth study

Front Endocrinol (Lausanne). 2023 Oct 19:14:1281649. doi: 10.3389/fendo.2023.1281649. eCollection 2023.

Authors

Xiaofeng Xu^{1

2

3}, Dongsheng Peng^{1

2

4

5}, Bowei Zhou^{1

2}, Kaijin Lin^{1

2}, Siyi Wang^{1

2}, Wei Zhao^{1

2}, Minqian Zheng^{1

2

6}, Jin Yang^{1

2

6}, Jianbin Guo^{1

2

6}

Affiliations

¹ Fujian Provincial Engineering Research Center of Oral Biomaterial, Fujian Medical University, Fuzhou, China.
² School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.
³ Department of Stomatology, Affiliated Hospital of Putian University, Putian, China.
⁴ Department of Stomatology, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, China.
⁵ Department of Stomatology, Fujian Obstetrics and Gynecology Hospital, Fuzhou, China.
⁶ Research Center of Dental and Craniofacial Implants, Fujian Medical University, Fuzhou, China.

Abstract

Objective: The purpose of this study was to prospectively evaluate the efficacy of a demineralized dentin matrix (DDM) in decreasing the initial inflammatory response of the gingiva and facilitating the repair and regeneration of soft tissue in alveolar ridge preservation.

Methods: This clinical study employed a split-mouth design. Fourteen patients with a total of forty-four sites underwent extraction and alveolar ridge preservation (ARP) procedures. A Bilaterally symmetrical extraction operation were conducted on the premolars of each patient. The experimental group received DDM as a graft material for ARP, while the control group underwent natural healing. Within the first month postoperatively, the pain condition, color, and swelling status of the extraction sites were initially assessed at different time points Subsequently, measurements were taken for buccal gingival margin height, buccal-lingual width, extraction socket contour, and the extraction socket area and healing rate were digitally measured. Additionally, Alcian Blue staining was used for histological evaluation of the content during alveolar socket healing.

Results: Both groups experienced uneventful healing, with no adverse reactions observed at any of the extraction sites. The differences in VAS pain scores between the two groups postoperatively were not statistically significant. In the early stage of gingival tissue healing (3 days postoperatively), there were statistically significant differences in gingival condition and buccal gingival margin height between the two groups. In the later stage of gingival tissue healing (7, 14, and 30 days postoperatively), there were statistically significant differences in buccal-lingual width, extraction socket healing area, and healing rate between the two groups. Furthermore, the histological results from Alcian Blue staining suggested that the experimental group may play a significant role in promoting gingival tissue healing, possibly by regulating inflammatory responses when compared to the control group.

Conclusion: The application of DDM in alveolar ridge preservation has been found to diminish initial gingival inflammation after tooth extraction. Additionally, it has shown the ability to accelerate early gingival soft tissue healing and preserve its anatomical contour.

Clinical trial registration: chictr.org.cn, identifier ChiCTR2100050650.

Keywords: alveolar ridge preservation; bone graft materials; demineralized dentin matrix; gingival healing; orthodontics.

Publication types

Clinical Study
Research Support, Non-U.S. Gov't

MeSH terms

Alcian Blue
Alveolar Process* / pathology
Alveolar Process* / surgery
Alveolar Ridge Augmentation* / methods
Bicuspid / surgery
Gingiva / surgery
Humans
Pain
Prospective Studies
Tooth Socket / pathology
Tooth Socket / surgery

Substances

Alcian Blue
Demineralized Dentin Matrix

Associated data

ChiCTR/ChiCTR2100050650

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. Project was supported by the Natural Science Foundation of Fujian Province, China (Grant No. 2020J01646, 2022J01764), Science and Technology Innovation Joint Foundation of Fujian Province, China (Grant No. 2020Y9031), Science and technology planning project of Fujian Provincial Health Commission (Grant No. 2022GGA041, Grant No. 2021GGB016), Startup Fund for scientific research, Fujian Medical University(Grant No.2021QH1133).