Long-term live cell imaging during differentiation of human iPSC-derived neurons

Jingqi Wang; Paul A Gleeson; Lou Fourriere

doi:10.1016/j.xpro.2023.102699

Long-term live cell imaging during differentiation of human iPSC-derived neurons

STAR Protoc. 2023 Dec 15;4(4):102699. doi: 10.1016/j.xpro.2023.102699. Epub 2023 Nov 7.

Authors

Jingqi Wang¹, Paul A Gleeson², Lou Fourriere³

Affiliations

¹ The Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3010, Australia.
² The Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3010, Australia. Electronic address: pgleeson@unimelb.edu.au.
³ The Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3010, Australia. Electronic address: lou.fourriere@unimeb.edu.au.

Abstract

Live-cell imaging is crucial to appreciate the dynamics and the complexity of cellular interaction processes. However, live-cell imaging of human neurons is challenging due to neuronal sensitivity. Here, we describe a long-term live-cell imaging protocol for neurons derived from human induced pluripotent stem cells. By using an IncuCyte live-cell imaging system, we have obtained information on neuronal dynamics during the different stages of neurogenesis. The protocol has also been developed to monitor the dynamics of the neuronal intracellular organelles. For complete details on the use and execution of this protocol, please refer to Wang et al.¹.

Keywords: Cell Biology; Cell Culture; Cell Differentiation; Microscopy; Neuroscience; Stem Cells.

MeSH terms

Cell Differentiation
Diagnostic Imaging
Humans
Induced Pluripotent Stem Cells*
Neurogenesis
Neurons